grubbs' test for removing outliers graphpad prism  (GraphPad Software Inc)

Journal: Frontiers in Immunology

Article Title: Cellular and transcriptional impacts of Janus kinase and/or IFN-gamma inhibition in a mouse model of primary hemophagocytic lymphohistiocytosis

doi: 10.3389/fimmu.2023.1137037

Figure Lengend Snippet: aIFNg, ruxolitinib, and combination treatment of Prf1 −/− mice infected with LCMV Armstrong. (A) Schematic representation of the experiment; Prf1 −/− mice infected with LCMV Armstrong (LCMV ARM) and treated with IFNg neutralizing antibody (aIFNg), ruxolitinib, or a combination of both agents (as described in the Methods) starting day 4 and continuing until day 8 p.i. Mice were then euthanized and multiple HLH parameters were evaluated on day 9 p.i. Naïve mice served as a negative control. (B) Number of red blood cells (RBC) (10 6 /μL), hemoglobin (HB) concentration (g/dL) and number of platelets (PLT) (10 3 /μL) in the various mouse cohorts. (C) Spleen weights depicted as a proportion of the final body weight. (D) Concentration of serum cytokines in the blood. Data points were pooled from two experiments and each data point represents one mouse. Outliers were removed using Grubb’s test (E) Representative images of hematoxylin and eosin-stained liver sections shown at a magnification of 20X; Each sample represents one liver section from one mouse. Sections from histological analysis were randomly chosen in a blinded fashion for inclusion in the Figure. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Outliers were removed with Grubb’s test using GraphPad Prism outlier calculator.

Techniques: Infection, Negative Control, Concentration Assay, Staining